- 品牌:聯(lián)祖
- 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
- 貨號(hào):LZ-H5381
- 發(fā)布日期: 2020-07-14
- 更新日期: 2025-01-15
產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
保存條件 | 低溫冷藏 |
品牌 | 聯(lián)祖 |
貨號(hào) | LZ-H5381 |
用途 | 公司產(chǎn)品僅用于科研 |
檢測(cè)方法 | elisa |
CAS編號(hào) | |
保質(zhì)期 | 詳見(jiàn)說(shuō)明書(shū) |
適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
檢測(cè)限 | 馬鈴薯、鹿、羊、雞、鴨、魚(yú)、人、大鼠、小鼠、豚鼠、倉(cāng)鼠、裸鼠、兔子、豬、犬、猴、馬、牛等 |
數(shù)量 | 60 |
英文名稱 | Human soluble tumor?necrosis factor?related apoptosis inducing ligand?(sTRAIL)?ELISA Kit |
包裝規(guī)格 | 48T/96T |
標(biāo)記物 | 詳見(jiàn)說(shuō)明書(shū) |
純度 | 詳見(jiàn)說(shuō)明書(shū)% |
樣本 | 詳見(jiàn)說(shuō)明書(shū) |
應(yīng)用 | 用于測(cè)定血清,血漿及相關(guān)液體等樣本。 |
是否進(jìn)口 | 是 |
產(chǎn)品名稱:人可溶性相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa定量檢測(cè)試劑盒48T/96T
英文名稱:Human soluble tumor necrosis factor related apoptosis inducing ligand (sTRAIL) ELISA Kit
規(guī)格 :48T/96T
主要成分:酶標(biāo)板,試劑,標(biāo)準(zhǔn)品等。
試劑盒種屬:馬鈴薯、鹿、羊、雞、鴨、魚(yú)、人、大鼠、小鼠、豚鼠、倉(cāng)鼠、裸鼠、兔子、豬、犬、猴、馬、牛等動(dòng)植物。
檢測(cè)目的:用于測(cè)定血清,血漿及相關(guān)液體等樣本。產(chǎn)品僅用于科研例如適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本..
人可溶性相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa定量檢測(cè)試劑盒48T/96T英文名稱:Human soluble tumor necrosis factor related apoptosis inducing ligand (sTRAIL) ELISA KitELISA試劑為我司主營(yíng)產(chǎn)品之一,產(chǎn)品皆嚴(yán)格按照相關(guān)標(biāo)準(zhǔn)進(jìn)行 ,并經(jīng)過(guò)了嚴(yán)格地反復(fù)地檢測(cè),我們以嚴(yán)謹(jǐn)?shù)膽B(tài)度保證產(chǎn)品的質(zhì)量,從而保證您的實(shí)驗(yàn)順利進(jìn)行。產(chǎn)品供貨周期短,供貨及時(shí),且我司還提供完整的售前和售后服務(wù)。別名:人可溶性相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa檢測(cè)試劑盒產(chǎn)品規(guī)格:48T/96T。
操作流程如下:
(1)產(chǎn)品僅用于科研待測(cè)樣品孔中每孔加入待測(cè)樣品100μl,每種樣品設(shè)3個(gè)平行孔;設(shè)兩個(gè)陰性對(duì)照孔,每孔加未處理組的細(xì)胞裂解液100μl;另設(shè)一個(gè)空白對(duì)照孔,加入純細(xì)胞裂解液100μl。
(2)酶標(biāo)板置4℃,包被過(guò)夜。
(3)洗板:吸干孔內(nèi)反應(yīng)液,用洗滌液過(guò)洗一遍(將洗滌液注滿板孔后,即甩去),之后將洗滌液注滿板孔,浸泡1-2分鐘,間歇搖動(dòng)。甩去孔內(nèi)液體后在吸水紙上拍干。重復(fù)洗滌3-4次。
(4)陰性對(duì)照孔每孔加入PBS 50μl,樣品孔及空白孔每孔加入1:500稀釋的兔抗人AIF抗體工作液50μl?!?/span>
(5)酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi),孵育60min。
(6)洗板,同(4)。
(7)每孔加1:5000稀釋的HRP-標(biāo)記的山羊抗兔抗體工作液 100μl?!?/span>
(8)酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi),孵育60min。
(9)洗板,同(4)。
(10)每孔加TMB顯色液 100μl,輕輕混勻10s,置37℃暗處反應(yīng)15-20min?!?/span>
(11)每孔加100μl 2mol/L H2SO4終止反應(yīng)?!?/span>
(12)分別測(cè)450nm 吸光值W1和630nm吸光值W2,zui終測(cè)得的OD值為兩者之差(W1-W2),以減少由容器上的劃痕或指印等造成的光干擾。
(13)數(shù)據(jù)處理:在得出標(biāo)本(S)和陰性對(duì)照(N)的 OD值后,計(jì)算S/N值。S/N≥2.1為陽(yáng)性判定標(biāo)準(zhǔn)。
注意事項(xiàng):
1.從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔
*孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)*乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請(qǐng)避光保存。
7. 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號(hào)組分不得混用。
實(shí)驗(yàn)標(biāo)準(zhǔn)品圖:
40 ng/L,5號(hào)標(biāo)準(zhǔn)品,150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
20 ng/L,4號(hào)標(biāo)準(zhǔn)品,150μl的5號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
10 ng/L,3號(hào)標(biāo)準(zhǔn)品,150μl的4號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
5 ng/L,2號(hào)標(biāo)準(zhǔn)品,150μl的3號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
2.5 ng/L,1號(hào)標(biāo)準(zhǔn)品,150μl的2號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
以下是人可溶性相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa定量檢測(cè)試劑盒48T/96T的相關(guān)產(chǎn)品:
ATP5A抗體 HCRTR2 (Orexin receptor type 2) ELISA KITAlias: TNFRSF12A|Fn14|TNFRSF12|CD266|CD266antigen|FGF-inducible14|Fibroblastgrowthfactor-inducibleimmediate-earlyresponseprotein14|FN14tumornecrosisfactorreceptorsuperfamilymember12A|tumornecrosisfactorreceptorsuperfamily|member12A|TWEAKR|TweakR|Tweak-receptor|tweak-receptor|typeItransmembraneproteinFn14ELISAKitReactivity: HumanRange: 0.156-10ng/mlSensitivity: 0.094ng/ml
即刻早期基因ARC抗體 IL1F10(Interleukin-1 family member 10) ELISA KitAlias: TEC|PSCTK4|EC2.7.10|EC2.7.10.2|MGC126760|MGC126762|tyrosine-proteinkinaseTec|tecproteintyrosinekinaseELISAKitReactivity: HumanRange: 0.156-10ng/mlSensitivity: 0.094ng/ml
ADP核糖基化因子6抗體 ISLR (Immunoglobulin superfamily containing leucine-rich repeat protein) ELISA KITAlias: CDH6|K-Cadherin|cadherin6|type2|K-cadherin(fetalkidney)|cadherin|fetalkidney|cadherin-6|KCAD|K-cadherin|K-cadherin(fetalkidney)ELISAKitReactivity: HumanRange: 0.313-20ng/mlSensitivity: 0.188ng/ml
alpha 1腎上腺素能受體A抗體 IGFN1 (Immunoglobulin-like and fibronectin type III domain-containing protein 1) ELISA KITAlias: MC5R|MC5-R|MC-2|MC-2|MC5-R|melanocortin5receptor|melanocortinreceptor5|melanocortin-5receptorELISAKitReactivity: HumanRange: 0.781-50ng/mlSensitivity: 0.469ng/ml
腺苷酸激酶-1抗體 ADRA1A(Alpha-1A adrenergic receptor) ELISA KitAlias: MSH2|MSH2|COCA1|FCC1|HNPCC|HNPCC1|LCFS2|MutSproteinhomolog2|LCFS2|DNAmismatchrepairproteinMsh2|hMSH2|HNPCC1mutS(E.coli)homolog2(coloncancer|nonpolyposistype1)|LCFS2|mutShomolog2|coloncancer|nonpolyposistype1(E.coli)|MutSproteinhomolog2ELISAKitReactivity: HumanRange: 78.125-5000pg/mlSensitivity: 46.875pg/ml
調(diào)亡誘導(dǎo)因子抗體 IL1RL2 (Interleukin-1 receptor-like 2) ELISA KitAlias: ARC|ARG3.1|mArc|Activity-regulatedgene3.1proteinhomologELISAKitReactivity: HumanRange: 15.625-1000pg/mlSensitivity: 9.375pg/ml
間變型激酶抗體 ICOSLG (ICOS ligand) ELISA KITAlias: PDX1|IDX1|IPF1|Glucose-sensitivefactor|IDX-1GSF|Insulinpromoterfactor1|insulinpromoterfactor1|homeodomaintranscriptionfactor|Insulinupstreamfactor1|IPF1pancreas|duodenumhomeoboxprotein1|Islet|duodenumhomeobox-1|MODY4IUF1|pancreaticandduodenalhomeobox1|pancreatic-duodenalhomeoboxfactor1|PDX-1IPF-1|somatostatintranscriptionfactor1|Somatostatin-transactivatingfactor1|STF-1IUF-1ELISAKitReactivity: HumanRange: 15.625-1000pg/mlSensitivity: 9.375pg/ml
α-甲基酰基輔酶A消旋酶抗體 Anti-M3 AChR (Anti-M3 Acetylcholine Receptor Antibody) ELISA KitAlias: C19orf10|MYDGF|chromosome19openreadingframe10|Myeloid-derivedgrowthfactorELISAKitReactivity: HumanRange: 15.625-1000pg/mlSensitivity: 9.375pg/ml
緊張素Ⅱ抗體 IKBIP (Inhibitor of nuclear factor kappa-B kinase-interacting protein) ELISA KITAlias: UBE3A|ANCR|EPVE6AP|HPVE6A|ANCREPVE6AP|AS|CTCLtumorantigense37-2|E6AP|E6-AP|E6APubiquitin-proteinligase|HPVE6A|HumanpapillomavirusE6-associatedprotein|HumanpapillomavirusE6-associatedprotein|Oncogenicprotein-associatedproteinE6-AP|RenalcarcinomaantigenNY-REN-54|ubiquitinproteinligaseE3A|ubiquitin-proteinligaseE3AELISAKitReactivity: HumanRange: 0.313-20ng/mlSensitivity: 0.188ng/ml
緊張素Ⅱ受體1抗體 HAGHL (Hydroxyacylglutathione hydrolase-like protein) ELISA KITAlias: ENTPD1|ADPase|ATP-diphosphatase|CD39|NTPD1|ATPDase|CD39antigen|ecto-apyrase|Ecto-apyrase|Ecto-ATPdiphosphohydrolase1|Ecto-ATPase1|Ecto-ATPDase1|ectonucleosidetriphosphatediphosphohydrolase1|FLJ40921|FLJ40959|Lymphoidcellactivationantigen|NTPDase1|NTPDase-1ELISAKitReactivity: HumanRange: 78.125-5000pg/mlSensitivity: 46.875pg/ml
膜粘連蛋白 Ⅱ抗體 HYLS1 (Hydrolethalus syndrome protein 1 homolog) ELISA KITAlias: MAPK8|PRKM8|SAPK1|c-JunN-terminalkinase1|JNK1alphaproteinkinase|JNK1betaproteinkinase|JNK1JNK1A2|JNK-46|JUNN-terminalkinase|MAPkinase8|MAPK8|mitogen-activatedproteinkinase8|mitogen-activatedproteinkinase8isoformJNK1alpha1|mitogen-activatedproteinkinase8isoformJNK1beta2|PRKM8JNK|proteinkinaseJNK1|SAPK1JNK21B1|2|Stress-activatedproteinkinase1|Stress-activatedproteinkinaseJNK1ELISAKitReactivity: HumanRange: 0.313-20ng/mlSensitivity: 0.188ng/ml
水通道蛋白4抗體 IPMK (Inositol polyphosphate multikinase) ELISA KITAlias: CHIA|Lung-specificproteinTSA1902|AMCaseELISAKitReactivity: HumanRange: 0.156-10ng/mlSensitivity: 0.094ng/ml
淀粉樣肽前體蛋白抗體 HCAR2 (Hydroxycarboxylic acid receptor 2) ELISA KITAlias: AVPR1B|AVPR3|V1bR|VPR3|Antidiuretichormonereceptor1b|argininevasopressinreceptor1B|AVPRV1b|AVPRV3|AVPR3argininevasopressinreceptor3|pituitaryvasopressinreceptor3|V1bR|vasopressinV1breceptor|VasopressinV3receptor|VPR3ELISAKitReactivity: HumanRange: 0.313-20ng/mlSensitivity: 0.188ng/ml
水通道蛋白-2抗體 INPPL1 (Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2) ELISA KITAlias: LILRB2|CD85d|ILT4|LIR2|MIR10|CD8*ntigen-likefamilymemberD|CD85dantigen|Ig-liketranscript4|ILT-4|Immunoglobulin-liketranscript4|Leukocyteimmunoglobulin-likereceptor2|leukocyteimmunoglobulin-likereceptorsubfamilyBmember2|leukocyteimmunoglobulin-likereceptorsubfamilyBmember2solubleisoform|leukocyteimmunoglobulin-likereceptor|subfamilyB(withTMandITIMdomains)|LIR-2|subfamilyA(withTMdomain)|member6|member2|LILRA6|Monocyte|macrophageimmunoglobulin-likereceptor10ELISAKitReactivity: HumanRange: 0.156-10ng/mlSensitivity: 0.094ng/ml
細(xì)胞死亡調(diào)節(jié)蛋白抗體(凋亡、半胱胺酸蛋白酶激活抑制劑) HCAR3 (Hydroxycarboxylic acid receptor 3) ELISA KITAlias: HOGA1|DHDPSL|DHDPS-likeprotein|ProbableKHG-aldolase|Protein569272|Dihydrodipicolinatesynthase-like|Probable2-keto-4-hydroxyglutaRatealdolaseELISAKitReactivity: HumanRange: 0.313-20ng/mlSensitivity: 0.188ng/ml
人可溶性相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa定量檢測(cè)試劑盒48T/96TPathScan® Phospho-RSK1 (Ser380) Sandwich ELISA Kit1 Kit
PathScan® Total RSK1 Sandwich ELISA Kit1 Kit
PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit1 Kit
PathScan® Phospho-Syk (Tyr525/526) Sandwich ELISA Kit100 ul
MAFA (D2Z6N) Rabbit mAb1 Kit
PathScan® Total mTOR Sandwich ELISA Kit100 ul
BCAT2 (D8K3O) Rabbit mAb1 Kit
PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit100 ul