產地 | 進口、國產 |
保存條件 | 低溫冷藏 |
品牌 | 聯祖 |
貨號 | LZ-R3732 |
用途 | 公司產品僅用于科研 |
檢測方法 | elisa |
CAS編號 | |
保質期 | 詳見說明書 |
適應物種 | 詳見說明書 |
檢測限 | 馬鈴薯、鹿、羊、雞、鴨、魚、人、大鼠、小鼠、豚鼠、倉鼠、裸鼠、兔子、豬、犬、猴、馬、牛等 |
數量 | 47 |
包裝規格 | 48T/96T |
標記物 | 詳見說明書 |
純度 | 詳見說明書% |
樣本 | Anti endothelial?cell antibodies?(AECA)?in rat?ELISA Kit |
應用 | 用于測定血清,血漿及相關液體等樣本。 |
是否進口 | 是 |
大鼠抗內皮細胞抗體(AECA)elisa試劑盒48T/96T英文名稱:Anti endothelial cell antibodies (AECA) in rat ELISA KitELISA試劑為我司主營產品之一,產品皆嚴格按照相關標準進行 ,并經過了嚴格地反復地檢測,我們以嚴謹的態度保證產品的質量,從而保證您的實驗順利進行。產品供貨周期短,供貨及時,且我司還提供完整的售前和售后服務。別名:大鼠抗內皮細胞抗體(AECA)elisa檢測試劑盒產品規格:48T/96T。
產品名稱:大鼠抗內皮細胞抗體(AECA)elisa試劑盒48T/96T
英文名稱:Anti endothelial cell antibodies (AECA) in rat ELISA Kit
規格 :48T/96T
主要成分:酶標板,試劑,標準品等。
試劑盒種屬:馬鈴薯、鹿、羊、雞、鴨、魚、人、大鼠、小鼠、豚鼠、倉鼠、裸鼠、兔子、豬、犬、猴、馬、牛等動植物。
檢測目的:用于測定血清,血漿及相關液體等樣本。產品僅用于科研例如適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養上清、組織勻漿等標本..
實驗標準品圖:
40 ng/L,5號標準品,150μl的原倍標準品加入150μl標準品稀釋液
20 ng/L,4號標準品,150μl的5號標準品加入150μl標準品稀釋液
10 ng/L,3號標準品,150μl的4號標準品加入150μl標準品稀釋液
5 ng/L,2號標準品,150μl的3號標準品加入150μl標準品稀釋液
2.5 ng/L,1號標準品,150μl的2號標準品加入150μl標準品稀釋液
注意事項:
1.從冷藏環境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存。
2. 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果。
3. 各步加樣均應使用加樣器,并經常校對其準確性,以避免試驗誤差。一次加樣時間控制在5分鐘內,如標本數量多,推薦使用排槍加樣。
4. 請每次測定的同時做標準曲線,做復孔。如標本中待測物質含量過高(樣本OD值大于標準品孔
*孔的OD值),請先用樣品稀釋液稀釋一定倍數(n倍)后再測定,計算時請*乘以總稀釋倍數(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數為準.
8. 所有樣品,洗滌液和各種廢棄物都應按傳染物處理。
9. 本試劑不同批號組分不得混用。
操作流程如下:
(1)產品僅用于科研待測樣品孔中每孔加入待測樣品100μl,每種樣品設3個平行孔;設兩個陰性對照孔,每孔加未處理組的細胞裂解液100μl;另設一個空白對照孔,加入純細胞裂解液100μl。
(2)酶標板置4℃,包被過夜。
(3)洗板:吸干孔內反應液,用洗滌液過洗一遍(將洗滌液注滿板孔后,即甩去),之后將洗滌液注滿板孔,浸泡1-2分鐘,間歇搖動。甩去孔內液體后在吸水紙上拍干。重復洗滌3-4次。
(4)陰性對照孔每孔加入PBS 50μl,樣品孔及空白孔每孔加入1:500稀釋的兔抗人AIF抗體工作液50μl?!?/span>
(5)酶標板置37℃培養箱的濕盒內,孵育60min?!?/span>
(6)洗板,同(4)?!?/span>
(7)每孔加1:5000稀釋的HRP-標記的山羊抗兔抗體工作液 100μl?!?/span>
(8)酶標板置37℃培養箱的濕盒內,孵育60min。
(9)洗板,同(4)?!?/span>
(10)每孔加TMB顯色液 100μl,輕輕混勻10s,置37℃暗處反應15-20min?!?/span>
(11)每孔加100μl 2mol/L H2SO4終止反應?!?/span>
(12)分別測450nm 吸光值W1和630nm吸光值W2,zui終測得的OD值為兩者之差(W1-W2),以減少由容器上的劃痕或指印等造成的光干擾。
(13)數據處理:在得出標本(S)和陰性對照(N)的 OD值后,計算S/N值。S/N≥2.1為陽性判定標準。
以下是大鼠抗內皮細胞抗體(AECA)elisa試劑盒48T/96T的相關產品:
人卵巢顆粒細胞瘤anti- Collagen Type II antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人細胞anti- Collagen Type III antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
大鼠肺動脈平滑肌細胞anti- Collagen Type III(N-Terminal) antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
小鼠胰腺腺泡細胞anti- Collagen Type IV antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
小鼠逆轉錄病毒包裝細胞anti- Collagen Type VI antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人NK/T細胞細胞anti- Collagen Type VI antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
羅猴胎腎細胞anti- Collagen Type VIII antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
高轉移人細胞anti- Collagen Type XVI antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
白介素-2轉染耐VP16絨癌細胞anti- Collagen Type XXIII antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人口腔表皮樣癌細胞anti- COMMD1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人Wilms細胞anti- COMMD5 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人細胞Ocut-2C(干細胞庫保藏)anti- COMMD8 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人經典小細胞細胞anti- Complement factor B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人細胞anti- Complement factor B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Protein A+G purification
人回盲細胞anti- Complement factor H antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人慢性髓原細胞anti- COMT antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
人乳腺浸潤性導管癌旁皮膚細胞anti- Connexin-26 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
大鼠抗內皮細胞抗體(AECA)elisa試劑盒48T/96T麥芽糖假絲酵母Human SLC31A1(High affinity copper uptake protein 1) ELISA KitAlias SLC31A1|Highaffinitycopperuptakeprotein1|Coppertransporter1|hCTR1|Solutecarrierfamily31member1|COPT1|CTR1ELISAKitReactivity HumanRange 0.781-50ng/mlSensitivity 0.469ng/ml
假絲酵母(加詞待譯)Human AGMAT(Agmatinase, mitochondrial) ELISA KitAlias AGMAT|Agmatinase|mitochondrial|Agmatineureohydrolase|AUHELISAKitReactivity HumanRange 0.781-50ng/mlSensitivity 0.469ng/ml
斯巴達克畢赤酵母Human AKR1C1(Aldo-keto reductase family 1 member C1) ELISA KitAlias AKR1C1|Aldo-ketoreductasefamily1memberC1|Trans-1|2-dihydrobenzene-1|2-dioldehydrogenase|Indanoldehydrogenase|High-affinityhepaticbileacid-bindingprotein|HBAB|Dihydrodioldehydrogenase1|2|DD1|DD2|20-alpha-hydroxysteroiddehydrogenase|20-alpha-HSD|ChlordeconereductasehomologHAKRC|DDH|DDH1|C9|H-37|MBAB|2-ALPHA-HSDELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml
墨西哥畢赤酵母Human ACLY(ATP-citRate synthase) ELISA KitAlias ACLY|ATP-citRatesynthase|ATP-citRate(pro-S-)-lyase|ACL|CitRatecleavageenzyme|ATPCL|CLATPELISAKitReactivity HumanRange 1.56-100IU/mlSensitivity 0.938IU/ml
加利福尼亞接合擬威爾酵母Human YRDC (YrdC domain-containing protein, mitochondrial) ELISA KITAlias YRDCELISAKitReactivity HumanRange Required
清酒假絲酵母Human ITIH5L(Inter-alpha-trypsin inhibitor heavy chain H5-like protein) ELISA KitAlias ITIH5L|Inter-alpha-trypsininhibitorheavychainH5-likeprotein|Inter-alphainhibitorH5-likeprotein|UNQ6369|PRO21074ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml
指甲畢赤酵母Human EFNA1(Ephrin-A1) ELISA KitAlias EFNA1|EphrinA1|EFNA1|TNFalphainducedprotein4|LERK1|EFL1|B61|ECKLG|EPLG1|TNFAIP4|Tumornecrosisfactoralpha-inducedprotein4|TNFalpha-inducedprotein4|EPH-relatedreceptortyrosinekinaseligand1|LERK-1|ImmediateearlyresponseproteinB61ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml