- 品牌:聯祖
- 產地:進口、國產
- 貨號:LZ-R3703
- 發布日期: 2020-06-22
- 更新日期: 2025-01-22
產地 | 進口、國產 |
保存條件 | 低溫冷藏 |
品牌 | 聯祖 |
貨號 | LZ-R3703 |
用途 | 公司產品僅用于科研 |
檢測方法 | elisa |
CAS編號 | |
保質期 | 詳見說明書 |
適應物種 | 詳見說明書 |
檢測限 | 馬鈴薯、鹿、羊、雞、鴨、魚、人、大鼠、小鼠、豚鼠、倉鼠、裸鼠、兔子、豬、犬、猴、馬、牛等 |
數量 | 33 |
包裝規格 | 48T/96T |
標記物 | 詳見說明書 |
純度 | 詳見說明書% |
樣本 | Rat soluble?platelet endothelial?cell adhesion molecule 1?(sPECAM-1/sCD31)?ELISA Kit |
應用 | 用于測定血清,血漿及相關液體等樣本。 |
是否進口 | 是 |
訂購信息:
產品名稱 | 英文名稱 | 貨號 |
大鼠可溶性血小板內皮細胞粘附分子1(sPECAM-1/sCD31)elisa試劑盒48T/96T | Rat soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1/sCD31) ELISA Kit | LZ-R3703 |
大鼠可溶性血小板內皮細胞粘附分子1(sPECAM-1/sCD31)elisa試劑盒英文名稱:Rat soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1/sCD31) ELISA KitELISA試劑為我司主營產品之一,產品皆嚴格按照相關標準進行 ,并經過了嚴格地反復地檢測,我們以嚴謹的態度保證產品的質量,從而保證您的實驗順利進行。產品供貨周期短,供貨及時,且我司還提供完整的售前和售后服務。別名:大鼠可溶性血小板內皮細胞粘附分子1(sPECAM-1/sCD31)elisa檢測試劑盒產品規格:48T/96T。
試劑盒組成及試劑配制:
1. 產品僅用于科研酶聯板(Assay plate ):一塊(96孔)。
2. 標準品(Standard):2瓶(凍干品)。
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶。
4. 生物素標記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶。
5. 辣根過氧化物酶標記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶。
6. 生物素標記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶。
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍。
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4)。
樣本處理及要求:
1. 大鼠可溶性血小板內皮細胞粘附分子1(sPECAM-1/sCD31)elisa試劑盒48T/96T血清:全血標本請于室溫放置2小時或4℃過夜后于1000g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
2. 血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內于2 - 8°C 1000g離心20分鐘,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
3. 組織勻漿:用預冷的PBS (0.01M, pH=7.4)沖洗組織,去除殘留血液(勻漿中裂解的紅細胞會影響測量結果),稱重后將組織剪碎。將剪碎的組織與對應體積的PBS(一般按1:9的重量體積比,比如1g的組織樣品對應9mL的PBS,具體體積可根據實驗需要適當調整,并做好記錄。推薦在PBS中加入蛋白酶抑制劑)加入玻璃勻漿器中,于冰上充分研磨。為了進一步裂解組織細胞,可以對勻漿液進行超聲破碎,或反復凍融。將勻漿液于5000×g離心5~10分鐘,取上清檢測。
4. 細胞培養物上清或其它生物標本:1000g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
注:標本溶血會影響檢測結果,因此溶血標本不宜進行此項檢測。
公司產品優勢:
1,全:公司提供上萬種產品,涵蓋了生物試劑,elisa試劑盒,標準品,培養基,原裝耗材等,基本上各種科研所需產品在我司都能找到。
2,新:產品更新速度較快,基本上每周都有新產品出現。
3,優:產品質量好,零投訴。
4,品牌多:公司代理的試劑品牌:Amresco、Sigma、Fluka、Alfa、TCI、Merck等進口品牌,國產品牌我們價格更優,我們供應優級純,分析純,化學純,試劑級,基準試劑,實驗純,教學試劑,高純試劑,色譜純,光譜純,電子純等試劑級別(可根據客戶需求定制)。
5,售后:我公司具有優質的技術團隊,產品一旦售出,產品僅用于科研實驗過程中遇到困難可提供在線技術咨詢。使您使用產品時沒有任何的后顧之憂。
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